| Titre |
Production of transgenic beef cattle rich in n-3 PUFAs by somatic cell nuclear transfer |
| Auteurs |
ADOLIGBE Camus Mahougnon [4],
|
| Journal: |
Biotechnol Lett |
| Catégorie Journal: |
Internationale |
| Impact factor: |
1.639 |
| Volume Journal: |
37 |
| DOI: |
DOI 10.1007/s10529-015-1827-z |
| Resume |
Objectives Omega-3 polyunsaturated fatty acids (n-
3 PUFAs) are beneficial to human health. However,
the n-3 PUFAs contents of the livestock meat that we
consume daily are relatively low. Utilization of
transgenic technology to increase n-3 PUFAs contents
in livestock may solve this problem.
Results The omega-3 fatty acid desaturase (FAD3),
encoded by fat1 gene derived from Caenorhabditis
elegans (C. elegans), converts omega-6 polyunsaturated
fatty acids (n-6 PUFAs) to n-3 PUFAs. In the study,
a plasmid containing the codon-optimized C. elegans
fat1 gene (mfat1) was constructed and used to produce
transgenic beef cattle by somatic cell nuclear transfer.
Fourteen transgenic calves were obtained, and the
ratio of n-6 to n-3 PUFAs in the transgenic calvesdecreased from 5.33: 1 to 0.95: 1 compared with
negative controls.
Conclusions Our results demonstrated that the
codon-optimized C. elegans mfat1 gene can be
functionally expressed in the beef cattle and converts
n-6 PUFAs to n-3 PUFAs. |
| Mots clés |
Fatty acid desaturase ! Fat1 gene ! n-3
Polyunsaturated fatty acids ! Somatic cell nuclear
transfer ! Transgenic cattle |
| Pages |
1565 - 1571 |
| Fichier |
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