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The purpose of this study is to value the in vitro anti-sickling activity of aqueous
extracts of six plants used in the treatment of the sickle cell disease in Benin: Morinda lucida, Uvaria chamae, Lonchocarpus cyanescens, Croton zambesicus, Raphiostylis
beninensis and Xylopia aethiopica. A decrease in the rate of sickling was observed after
incubation of erythrocytes with plant extracts and 2% sodium metabisulfite as compared to
incubation with 0.9% NaCl. The results of the study about the inhibitory properties of the
SS erythrocytes sickling by the aqueous extract of the mixture of Raphiostylis beninensis,
Croton zambesicus, Lonchocarpus cyanescens, Uvaria chamae, Morinda lucida and
Xylopia aethiopica show that it has an obvious antisickling effect in vitro according to our
experimental conditions (sickling inhibition rate > 50%). It is the same with aqueous
extracts of Uvaria chamae, Morinda lucida and Xylopia aethiopica which inhibit more than
45% of SS erythrocytes sickling. Antisickling effects of those last plants on AS and SS
erythrocytes are otherwise highly correlated ("r " is respectively equal to 0,88; 0,88 and
0,66). The extract of Raphiostylis beninensis, Croton zambesicus and Lonchocarpus
cyanescens have relatively less effect (inhibition rate < 21%) particularly on SS
erythrocytes sickling and a correlation coefficient "r" respectively equal to -0,2353; 0,3959
and 0,723. Our results point out that these six plants have, at different doses, an obvious
antisickling effect in vitro and support the interest of their use in the traditional treatment of
sickle cell disease. |