Publications Scientifiques

[ Article ] Chemical variation of essential oil constituents of Ocimum gratissimum L. from Benin, and impact on antimicrobial properties and toxicity against Artemia salina leach

Date de soumission: 23-01-2017
Année de Publication: 2012
Entité/Laboratoire Laboratoires de Chimie Organique Phisyque et de Synthèse (LRCOPS)
Document type : Article
Discipline(s) : Chimie
Titre Chemical variation of essential oil constituents of Ocimum gratissimum L. from Benin, and impact on antimicrobial properties and toxicity against Artemia salina leach
Auteurs KPADONOU-KPOVIESSI Bénédicta [1], Yayi Ladekan Eléonore [2], KPOVIESSI Dossou Sika Salomé [3], Gbaguidi A. Fernand [4], YEHOUENOU BONIFACE B. [5], QUETIN-LECLERCQ Joëlle [6], FIGUEREDO GILLES [7], MOUDACHIROU Mansourou [8], ACCROMBESSI COFFI GEORGES [9],
Journal: Chemistry & Biodiversity
Catégorie Journal: Internationale
Impact factor: 0
Volume Journal: 9
DOI:
Resume o determine the period of harvest that optimizes the antimicrobial activities of the essential oil of Ocimum gratissimum L. from Benin, aerial plant parts were collected at two vegetative stages (pre- and full-flowering) and three sampling times (7 am, 1 pm, and 7 pm). Extraction by hydrodistillation yielded between 0.65 and 0.78% of essential oils. Characterization of the oils by GC-FID and GC/MS analysis revealed the presence of monoterpenes (87.26-93.81%), sesquiterpenes (5.57-11.34%), and aliphatic compounds (0.15-0.18%), with p-cymene (1; 28.08-53.82%), thymol (2; 3.32-29.13%), γ-terpinene (3; 1.11-10.91%), α-thujene (4; 3.37-10.77%), and β-myrcene (5; 4.24-8.28%) as major components. Two chemotypes were observed, i.e., a p-cymene/thymol and a p-cymene chemotype, for plants harvested at 7 am for the former and at 1 pm or 7 pm for the latter, respectively. The oils were fungicidal against Candida albicans, with the sample from full-flowering plants collected at 7 am being the most active (MIC = 0.06±0.00 mg/ml). The chemical variation of the oils also influenced the antimicrobial effect against Staphylococcus aureus; the most active oil was obtained from plants at the pre-flowering stage collected at 7 am (MIC=0.24±0.01 mg/ml). Escherichia coli was insensitive to the chemical variation of the oils (MICs of ca. 0.48±0.02 mg/ml for all oils). Moreover, the essential oils showed low toxicity against Artemia salina Leach larvae, with LC(50) values in the range of 43-146 μg/ml. This is the first study of the interaction between the daytime of collection and vegetative stage of the plants and the antimicrobial properties and toxicity of the essential oil of O. gratissimum from Benin.
Mots clés Ocimum gratissimum; antimicrobial activities; Benin
Pages 139 - 150
Fichier (PDF)

Publications par entité

  • Centre de Formation et de Recherche en matière de Population (CEFORP) (0)
  • Centre Interfacultaire de Formation et de Recherche en Environnement pour le Développement Durable (CIFRED) (0)
  • Chaire Internationale en Physique Mathématique et Applications (CIPMA-Chaire UNESCO) (0)
  • Ecole Doctorale Pluridisciplinaire <<Espaces, Culture et Développement>> (0)
  • Ecole Nationale d'Administration et de Magistrature (ENAM) (0)
  • Ecole Nationle d'Economie Appliquée et de Management (0)
  • Ecole Normale Supérieure (0)
  • Ecole Polytechnique d'Abomey-Calavi (EPAC) (0)
  • Faculté de droit et de Science Politique (FADESP) (0)
  • Faculté des Lettres et Sciences Humaines (FLASH) (0)
  • Faculté des Lettres, Langues, Arts et Communications (FLLAC) (0)
  • Faculté des Sciences Agronomiques (0)
  • Faculté des Sciences de la Santé (FSS) (0)
  • Faculté des Sciences Economiques et de Gestion (FASEG) (0)
  • Faculté des Sciences et Techniques (FAST) (0)
  • Faculté des Sciences Humaines et Sociales (FASHS) (0)
  • Institut de Formation et de Recherche en Informatique (IFRI) (0)
  • Institut de Géographie de l'Aménagement du territoire et de l'Environnement (IGATE) (0)
  • Institut de Mathématiques et de Sciences Physiques (IMSP) (0)
  • Institut National de l'Eau (INE) (0)
  • INSTITUT NATIONAL DE LA JEUNESSE DE L’EDUCATION PHYSIQUE ET SPORT(INJEPS) (0)
  • Institut National des Métiers d'Art d'Archéologie et de la Culture (INMAAC) (0)
  • Institut National Médico Sanitaire ( INMeS) (0)
  • Institut Régional de Santé Publique (IRSP) (0)

Publications par sexe

  • Femme( 1400 )
  • Homme (