||Densitometric HPTLC quantification of asiaticoside isolated from Centella asiatica (L.) Urb (Apiaceae) of Benin
GANSE Hippolyte ,
Gbaguidi A. Fernand ,
AGBANI Pierre O. ,
JOSSE Roger G. ,
MOUDACHIROU MANSOUROU ,
Sinsin Brice A. ,
Quetin-Leclercq Joëlle ,
AMINOU Taofiki ,
||Int. J. Biol. Chem. Sci.
||Asiaticoside isolated from Centella asiatica has been found through in vitro test to serve as an active
agent of healing on wounds. To quantify this compound in Centella asiatica cultivated in Benin, a new, simple
and rapid High-Performance Thin-Layer Chromatography (HPTLC) method was developed and validated for
its selectivity, its recovery, and its repeatability. Compounds have been separated on silica gel 60F254 plates
with ethyl acetate/methanol/water (100/25/10, v/v/v), as mobile phase. The detection was done by densitometry
scanning at λ = 600 nm and the calibration plots showed that the graphical response was linear and dependent
on the quantity in the range of 2.50- 12.50 µg, with good values of R² = 0.998. This method is repeatable and
precise with relative standard deviations between1.42 and 5.92% for the intra-day tests and between 1.27 and
6.57% for the inter-day tests for the quantities belonging to an interval of 2.50 and 12.50 µg. The limits of
detection and quantification were 0.317 and 1.05 µg respectively. The quantity of asiaticoside found was
0.24% in the alcoholic extract.
||HPTLC, Centella asiatica, Asiaticoside, Wounds.
||857 - 863