||Volatile oil composition and antiproliferative activity of Hyptis spicigera Lam against human breast adenocarcinoma cells MCF-7
Bogninou-Agbidinoukou G. Sophie ,
YEDOMONHAN HOUNNANKPON ,
AVLESSI FÉLICIEN ,
SOHOUNHLOUE KOKO CODJO DOMINIQUE ,
Chalard Pierre ,
CHALCHAT Jean C. ,
||Research Journal of Chemical Sciences
||This study reported the chemical composition and the evaluation of the in vitro antiproliferative activity of volatile extracts of
Hyptis spicigera (HS) on breast cancer cells MCF-7. Essential oils (EO) HS1 and HS2 extracted from leafy stems of H.
spicigera by hydrodistillation after different harvesting periods were characterized by gas chromatography/flame ionization
detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS). The cytotoxicity of these volatile extracts was
tested in order to assess their antiproliferative activity on cancer cells MCF-7 by the resazurin test. Chromatographic
analysis revealed several major compounds depending on the harvest period of vegetable. Sample HS1 was characterized by
-pinene (16.9%), sabinene (13.8%), -pinene (9.6%) and 1,8-cineole (9.6%), while HS2 was potentially rich in -
caryophyllene (53.2%), caryophyllene oxide (5.4%) and -humulene (3.8%). Biological investigations performed have shown
that HS1 and HS2 were cytotoxic against MCF-7 cells. Inhibitory concentrations values (IC50) determined for this purpose
were 170 and 84 μg/mL respectively for HS1 and HS2. Major compounds of the EO should be responsible for this inhibitory activity.
||Hyptis spicigera, volatile extract, MCF-7, -caryophyllene, -pinene, Benin.
||27 - 31