Publications Scientifiques

[ Article ] In vitro biological effects of two anti-diabetic medicinal plants used in Benin as folk medicine

Date de soumission: 06-02-2017
Année de Publication: 2013
Entité/Laboratoire Laboratoire d'Etude et de Recherche en Chimie Appliquée (LERCA)
Document type : Article
Discipline(s) : Chimie
Titre In vitro biological effects of two anti-diabetic medicinal plants used in Benin as folk medicine
Auteurs BOTHON FIFA THEOMAINE DIANE [1], Debiton Eric [2], Félicien Avlessi Félicien [3], Forestier Christian [4], Teulade Jean-Claude [5], SOHOUNHLOUE KOKO CODJO DOMINIQUE [6],
Journal: Complementary & Alternative Medecine
Catégorie Journal: Internationale
Impact factor: 0
Volume Journal: 13
DOI:
Resume Background: Extracts from Polygonum senegalensis (Polygonaceae) and Pseudocedrela kotschyi (Meliaceae) are two important traditionally used medicinal plants in rural Benin to treat many diseases and notably type 2 diabetes. The aim of the study was to investigate the α-glucosidase inhibition, antioxidant and antibacterial activities of those plants extract: Polygonum senegalensis leaves, and Pseudocedrela kotschyi root. Methods: Hydro-alcoholic (50%) extracts were analyzed for their phytochemical content and tested for their inhibition potency on α-glucosidase from Saccharomyces cerevisiae. Antioxidant activities were assessed using the DPPH, ORAC, FRAP and DCFH-DA (cell based) assay. Finally, the antibacterial activity was evaluated using MIC determination on four Gram-positive cocci (Bacillus subtilis, Clostridium difficile, Enterococcus faecalis, Staphylococcus aureus), three Gram-negative bacilli (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae), and the yeast Candida albicans. Results: Each extract presented significant α-glucosidase inhibition and antioxidant activities. Polygonum senegalensis leaf extracts were the most active in each in vitro assay with an IC50 = 1.5 μg/ml for α-glucosidase inhibition and an IC50 = 6.8 μg/ml for DPPH scavenging, - 4.5 μmol Fe II/g of dry matter - 9366 μmol Trolox / g DW - for FRAP and ORAC values, respectively. IC50 = 2.3 μg GA / ml for DCFH-DA assay. Concerning its antibacterial activity, a growth inhibitory effect was observed only against three Gram negative bacilli: B. subtilis, E. faecalis, S. aureus and the yeast C. albicans at high concentration. Conclusion: The results showed that the semi alcoholic extract of the two studied plants possess α-glucosidase inhibitory activity, antioxidant potency, and low antibacterial effect.
Mots clés Polygonum senegalensis, Pseudocedrela kotschyi, α-glucosidase, Antioxidant, Antibacterial
Pages 1 - 8
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