||Background: Extracts from Polygonum senegalensis (Polygonaceae) and Pseudocedrela kotschyi (Meliaceae) are two
important traditionally used medicinal plants in rural Benin to treat many diseases and notably type 2 diabetes. The
aim of the study was to investigate the α-glucosidase inhibition, antioxidant and antibacterial activities of those
plants extract: Polygonum senegalensis leaves, and Pseudocedrela kotschyi root.
Methods: Hydro-alcoholic (50%) extracts were analyzed for their phytochemical content and tested for their
inhibition potency on α-glucosidase from Saccharomyces cerevisiae. Antioxidant activities were assessed using the
DPPH, ORAC, FRAP and DCFH-DA (cell based) assay. Finally, the antibacterial activity was evaluated using MIC
determination on four Gram-positive cocci (Bacillus subtilis, Clostridium difficile, Enterococcus faecalis, Staphylococcus
aureus), three Gram-negative bacilli (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae), and the yeast
Results: Each extract presented significant α-glucosidase inhibition and antioxidant activities. Polygonum
senegalensis leaf extracts were the most active in each in vitro assay with an IC50 = 1.5 μg/ml for α-glucosidase
inhibition and an IC50 = 6.8 μg/ml for DPPH scavenging, - 4.5 μmol Fe II/g of dry matter - 9366 μmol Trolox / g DW
- for FRAP and ORAC values, respectively. IC50 = 2.3 μg GA / ml for DCFH-DA assay. Concerning its antibacterial
activity, a growth inhibitory effect was observed only against three Gram negative bacilli: B. subtilis, E. faecalis, S.
aureus and the yeast C. albicans at high concentration.
Conclusion: The results showed that the semi alcoholic extract of the two studied plants possess α-glucosidase
inhibitory activity, antioxidant potency, and low antibacterial effect.