| Titre |
Bovine GDF10 gene polymorphism analysis and its association with body measurement traits in Chinese indigenous cattle |
| Auteurs |
ADOLIGBE Camus Mahougnon [1],
|
| Journal: |
Mol Biol Rep. |
| Catégorie Journal: |
Internationale |
| Impact factor: |
2.506 |
| Volume Journal: |
39 |
| DOI: |
DOI 10.1007/s11033-011-1188-1 |
| Resume |
The objective of this research was to detect
bovine GDF10 gene polymorphism and analyze its association
with body measurement traits (BMT) of animals
sampled from 6 different Chinese indigenous cattle populations.
The populations included Xuelong (Xl), Luxi (Lx),
Qinchuan (Qc), Jiaxian red (Jx), Xianang (Xn) and Nanyang
(Ny). Blood samples were taken from a total of 417 female
animals stratified into age categories of 12–36 months.
Polymerase chain reaction–single strand conformation
polymorphism (PCR–SSCP) was employed to find out
GDF10 single polymorphism nucleotide (SNPs) and explore
their possible association with BMT. Sequence analysis of
GDF10 gene revealed 3 SNPs in total: 1 in exon1 (G142A)
and 2 in exon3 (A11471G, and T12495C). G142A and
T12495C SNPs are both synonymous mutation. They
showed 2 genotypes namely respectively (GG, GA) and (PP
and PB). A11471G SNP is a missense mutation leading to
the change of Alanine to Threonine amino acid. It showed
three genotypes namely AA, BB and AB. Analysis of
association of polymorphism with body measurement traits
at the three locus showed that there were significant effects on BMT in Qc, Jx and Ny cattle population. These results
suggest that the GDF10 gene might have potential effects on
body measurement traits in the above mentioned cattle
populations and could be used for marker-assisted selection. |
| Mots clés |
Chinese indigenous cattle, GDF10, Body
measurement traits, SNPs |
| Pages |
4067 - 4075 |
| Fichier |
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