Publications Scientifiques

[ Article ] Genetic variability of cultivated cowpea in Benin assessed by random amplified polymorphic DNA

Date de soumission: 13-03-2017
Année de Publication: 2008
Entité/Laboratoire Laboratoire de Génétique Ecologique
Document type : Article
Discipline(s) : Génétique et Amélioration des Plantes
Titre Genetic variability of cultivated cowpea in Benin assessed by random amplified polymorphic DNA
Auteurs ZANNOU A. S. AFIO [1], KOSSOU KODJO DANSOU [2], AHANCHEDE ADAM [3], ZOUNDJIHEKPON JEANNE [4], Agbicodo E. [5], STRUI P. C. [6], SANNI AMBALIOU [7],
Journal: African Journal of Biotechnology
Catégorie Journal: Internationale
Impact factor: 0.71
Volume Journal: 7
DOI:
Resume Characterization of genetic diversity among cultivated cowpea [Vigna unguiculata (L.)Walp.] varieties is important to optimize the use of available genetic resources by farmers, local communities, researchers and breeders. Random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic diversity in 70 cowpea accessions collected throughout Benin. Nine random primers were screened on 24 accessions to assess their ability to reveal polymorphisms in cowpea and four of them were selected for use in characterizing the total sample. A total of 32 amplified bands were generated by the four primers. The number of loci detected varied from 5 to 11. RAPD profiles were analysed and amplified polymorphic DNA fragments were used to construct a dendrogram, clustering the accessions into nine groups at a similarity index of 71% based on the Unweighted Pair –Group Method using Arithmetic Averages. The genetic diversity among the cowpea cultivars investigate was large and the RAPD proved to be a useful technique to characterize it. Based on the molecular variance, the fixation index suggests a large differentiation of cowpea cultivars in Benin.
Mots clés Characterization of genetic diversity among cultivated cowpea [Vigna unguiculata (L.)Walp.] varieties is important to optimize the use of available genetic resources by farmers, local communities, researchers and breeders. Random amplified polymorphic DNA
Pages 4407 - 4414
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