| Titre |
Expressive Modulation of the Cyp7a1 and Ibat Protein by Cholesterol and its Contribution to the Maintenance of the Normal Cholesterolemia in Mouse |
| Auteurs |
Attakpa Sèlidji Eugène [1],
Djibril Naguibou [2],
Ahokpè Mélanie [3],
SEZAN ALPHONSE [4],
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| Journal: |
Journal of Physiology and Pharmacology Advances |
| Catégorie Journal: |
Internationale |
| Impact factor: |
0 |
| Volume Journal: |
2 |
| DOI: |
|
| Resume |
The aim of our study is to prove the existence of a very coordinated system of regulation be-tween the route of excretion of taurocholic acid and cholesterol. We proceed to follow up phys-iologically animals, with no differences in water and food consumptions between the control group and the group of 5% diet of cholesterol. On the other hand dieresis has increased in mice with 5% diet of cholesterol. Cholesterol induced in the liver the transcribed rate of Cholesterol 7α-hydroxylaze (CYP7A1), limiting enzyme of bile acids synthesis. What’s more, expression of CYP7A1 encoding gene is induced in mice with 5% diet of cholesterol. Also we noticed an in-crease of distribution and wealth expression related to the RNA and CYP7A1 in the liver report-ed by Northern Blotting. According to ileum role in intestinal re-absorption of bile acids, our interest was focused on the ileac transportation: the standard expression of Ileal Bile Acid Transporter (IBAT) has strongly reduced; in mice with rich diet of cholesterol the Western Blot-ting of proteins has quantitatively reduced. IBAT’s oppression can be explained by the diminu-tion of the ileac transportation of taurocholic acid (TCA).It results an increase of fecal flight of TCA in mice with a rich diet of cholesterol. |
| Mots clés |
Bile acids, cholesterol, taurocholic acid, CYP7A1, IBAT, Mouse |
| Pages |
243 - 249 |
| Fichier |
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