||Modulation of TH1 differentiation Peroxisome Proliferator-Activated Receptorα-Independent Manner.
Attakpa Sèlidji Eugène ,
SEZAN ALPHONSE ,
BABA-MOUSSA LAMINE SAÏD ,
Seri Bialli ,
||International Journal of Scientific Research
||The influence of PPARα on numerous biological activities arises through its ability to carry out gene regulation. We examined the effects of PPARα on the expression of on key transcription factors involved in Th cell differentia-tion, T-bet, and determined whether PPARα mediates its effects. The T-cells from PPARα null mice secreted higher IFNγ and lower IL-2 concentrations than WT T-cells. We demonstrate that PPARα regulates the expression of these cytokines by CD4+ T cells, through its ability to negatively regulate TH1 differentiation via the transcription of T-bet both at mRNA and protein levels. T-cells from PPARαnull mice expressed higher p38 phosphorylation than WT T-cells. T-bet expression in CD4+ T cells was determined to be in-fluenced by p38 mitogen-activated protein (MAP) kinase activation. The presence of WY14,643, PPARα suppressed the phosphorylation of p38 MAP kinase in both the cell types. The pharmacological inhibitors of MAP kinases also downregulated T-bet in T-cells.
||T-cells, MAPK, mouse, PPAR
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