| Titre |
PPAR-{alpha} ROLE IN OBESITY-DIABETES IN MICE |
| Auteurs |
Attakpa Sèlidji Eugène [1],
SEZAN ALPHONSE [2],
Seri Bialli [3],
|
| Journal: |
Acta Endocrinologica (Buc) |
| Catégorie Journal: |
Internationale |
| Impact factor: |
0.3 |
| Volume Journal: |
4 |
| DOI: |
Doi: 10.4183/aeb. 11, 533-542. |
| Resume |
Background. Peroxisome proliferator–activated receptors (PPAR)α and γ are ligand-activated transcription factors and members of the nuclear hor-mone receptor superfamily that regulate the metabolism of glucose and li-pids.
Aim. This study investigated the effects of PPARα deficiency on body weight in wild type and PPAR-αnull mice.
Materials and methods. The study was performed on male wild type (WT) mice and homozygous PPAR-αnull (PPARα-knockout) mice of C57BL/6J ge-netic background. The mRNA expression was quantitatively analyzed by the real time of polymerase chain reaction (RTPCR). Liver TG content was measured by using a commercially available kit. Serum triglyceride (TG) content was measured using enzymatic methods. Serum insulin was deter-mined using an ELISA kit. Serum glucose was determined by the glucose oxidase method using a glucose analyser.
Results. Compared with WT, PPAR-αnull mice had high relative adipose tis-sue weight. These mice exhibited high adiposity state. PPAR-αnull mice also expressed high adiponectin and leptin mRNA levels compared to wild type animals.
The PPAR-αnull mice had significantly higher body weight than WT.
Hepatic TG and FFA were higher in PPAR- αnull mice as compared to WT animals. PPAR-αnull mice had a high accumulation of TG and FFA in the plasma. Serum insulin concentrations and its pancreatic mRNA transcripts were downregulated in PPAR-αnull mice, suggesting that PPARα gene dele-tion contributes to low insulin gene transcription.
We have reported that PPARα deficiency leads to hypoglycaemia in mice.
Conclusion. It is suggested a role of PPARα in obesity-diabetes in mice by studying PPARα-knockout mice. |
| Mots clés |
PPARα, Insulin,
adiponectin mRNA, leptin mRNA, hypoglycaemia,
free fatty acids, triglyceride. |
| Pages |
533 - 542 |
| Fichier |
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