| Titre |
Role of the genes involved in the transport of bile acids in the liver and kidneys in mice |
| Auteurs |
Attakpa Sèlidji Eugène [1],
Djibril Naguibou M [2],
BABA-MOUSSA FARID [3],
YESSOUFOU Ganiou [4],
SEZAN ALPHONSE [5],
|
| Journal: |
Journal of Basic and Clinical Physiology and Pharmacology |
| Catégorie Journal: |
Internationale |
| Impact factor: |
0 |
| Volume Journal: |
24 |
| DOI: |
|
| Resume |
Background: Bile acids are synthesized in the liver from
cholesterol.
Methods: This study investigated the impact and expression of different carriers of bile acid in the liver and
kidneys. Eight-week-old male mice were used, which were
fed for 15 days and divided into two groups: 15 mice fed with standard diet (control group) and another 15 mice fed with a rich diet of 5 % cholesterol (second group). Bile acid dosage was based on their oxidation by 7 α hydroxylsteroid
dehydrogenize. The mRNA expression was quantitatively
analyzed by the real time of polymerase chain reaction (RT-PCR), and the expression of the renal carrier bile acid protein was analyzed by Western blot.
Results and conclusions: The expression of bile salt export
pump involved in the uptake of bile acids in the basolateral
membrane of hepatocytes revealed no differences between the two groups of mice. However, the expression of multidrug resistance-associated protein 2 was reduced
in mice of the second group. Moreover, the expressions of
organic anion transporting polypeptide 4, organic anion
transporting polypeptide 1, and sodium taurocholate cotransporting polypeptide (Ntcp) involved in the uptake of
bile acids in the apical pole of hepatocytes are suppressed
in mice of the second group. The expression of multidrug
resistance-associated protein 3 involved in the secretion of
bile acids in the apical membrane of hepatocytes revealed
no significant differences between the two groups. In mice
of the second group, blood concentration of bile acids on
the last day was increased. In those mice, the expression
of intestinal bile acid transporter was reduced in the kidneys compared with the control mice. |
| Mots clés |
bile acid; kidneys; liver; mouse. |
| Pages |
97 - 103 |
| Fichier |
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