| Titre |
Expression and role of the genes involved in the transport of bile acids in the liver and kidneys in mice |
| Auteurs |
ATTAKPA S. EUGÈNE [1],
NAGUIBOU Djibril [2],
BABA-MOUSSA FARID A. KADER SAÏD [3],
YESSOUFOU ABDOU GANIOU [4],
SEZAN ALPHONSE [5],
|
| Journal: |
J Basic Clin Physiol Pharmacol |
| Catégorie Journal: |
Internationale |
| Impact factor: |
0.759 |
| Volume Journal: |
24 |
| DOI: |
DOI 10.1515/jbcpp-2012-0024 |
| Resume |
Background : Bile acids are synthesized in the liver from
cholesterol.
Method : This study investigated the impact and expression
of different carriers of bile acid in the liver and
kidneys. Eight-week-old male mice were used, which were
fed for 15 days and divided into two groups: 15 mice fed
with standard diet (control group) and another 15 mice
fed with a rich diet of 5 % cholesterol (second group). Bile
acid dosage was based on their oxidation by 7 α hydroxylsteroid
dehydrogenize. The mRNA expression was quantitatively
analyzed by the real time of polymerase chain
reaction (RT-PCR), and the expression of the renal carrier
bile acid protein was analyzed by Western blot.
Result : The expression of bile salt export pump involved
in the uptake of bile acids in the basolateral membrane
of hepatocytes revealed no differences between the two
groups of mice. However, the expression of multidrug
resistance-associated protein 2 was reduced in mice of the
second group. Moreover, the expressions of organic anion
transporting polypeptide 4, organic anion transporting
polypeptide 1, and sodium taurocholate co-transporting
polypeptide (Ntcp) involved in the uptake of bile acids in
the apical pole of hepatocytes are suppressed in mice of
the second group. The expression of multidrug resistanceassociated
protein 3 involved in the secretion of bile acids
in the apical membrane of hepatocytes revealed no significant
differences between the two groups. In mice of
the second group, blood concentration of bile acids on the
last day was increased. In those mice, the expression of intestinal bile acid transporter was reduced in the kidneys compared with the control mice. |
| Mots clés |
bile acid; kidneys; liver; mouse. |
| Pages |
97 - 103 |
| Fichier |
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