Publications Scientifiques

[ Article ] Expression and role of the genes involved in the transport of bile acids in the liver and kidneys in mice

Date de soumission: 24-10-2016
Année de Publication: 2013
Entité/Laboratoire Laboratoire de Biomembranes et Signalisation Cellulaire (LBSC)
Document type : Article
Discipline(s) : Endocrinologie, Métabolisme & Nutrition
Titre Expression and role of the genes involved in the transport of bile acids in the liver and kidneys in mice
Auteurs ATTAKPA S. EUGÈNE [1], NAGUIBOU Djibril [2], BABA-MOUSSA FARID A. KADER SAÏD [3], YESSOUFOU ABDOU GANIOU [4], SEZAN ALPHONSE [5],
Journal: J Basic Clin Physiol Pharmacol
Catégorie Journal: Internationale
Impact factor: 0.759
Volume Journal: 24
DOI: DOI 10.1515/jbcpp-2012-0024
Resume Background : Bile acids are synthesized in the liver from cholesterol. Method : This study investigated the impact and expression of different carriers of bile acid in the liver and kidneys. Eight-week-old male mice were used, which were fed for 15 days and divided into two groups: 15 mice fed with standard diet (control group) and another 15 mice fed with a rich diet of 5 % cholesterol (second group). Bile acid dosage was based on their oxidation by 7 α hydroxylsteroid dehydrogenize. The mRNA expression was quantitatively analyzed by the real time of polymerase chain reaction (RT-PCR), and the expression of the renal carrier bile acid protein was analyzed by Western blot. Result : The expression of bile salt export pump involved in the uptake of bile acids in the basolateral membrane of hepatocytes revealed no differences between the two groups of mice. However, the expression of multidrug resistance-associated protein 2 was reduced in mice of the second group. Moreover, the expressions of organic anion transporting polypeptide 4, organic anion transporting polypeptide 1, and sodium taurocholate co-transporting polypeptide (Ntcp) involved in the uptake of bile acids in the apical pole of hepatocytes are suppressed in mice of the second group. The expression of multidrug resistanceassociated protein 3 involved in the secretion of bile acids in the apical membrane of hepatocytes revealed no significant differences between the two groups. In mice of the second group, blood concentration of bile acids on the last day was increased. In those mice, the expression of intestinal bile acid transporter was reduced in the kidneys compared with the control mice.
Mots clés bile acid; kidneys; liver; mouse.
Pages 97 - 103
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