||Present study was realized in the aim to optimize of a renewed medium for the production of zooplankton with pig dung. In fact, the experiment was carried out in triplicate in plastic buckets, grouped together by three treatments (T1, T2 and T3) which were fertilized and a control (T0) during 27 days. The buckets were seeded with zooplankton with an initial density of 37 individual/l (Day0). From D12, those of T2 and T3 have sustained a partial and periodical renewal (50%) in the production medium. The treatment T3 was, in addition, fertilized periodically. The zooplankton density evolution is followed up through a sampling, every three days. The results have shown that the renewal followed with a periodical fertilization improved the zooplankton production (p ˂ 0.05) with rotifers predominance. Thus the treatment T3 media offered the best zooplanktonic average density (631 ± 440ind.l-1) as compared to the treatment T2 (392 ± 253ind.l-1). That renewal prevents the pollution of and the congestion of production medium. The adoption of this production technique of zooplankton permitted to get small sized live prey in mass which could be maintained during the period of larval rearing for the aquaculture hatcheries.