Publications Scientifiques

[ Article ] Activity of red wine polyphenols on endothelial nosynthase (eNOS)

Date de soumission: 07-06-2017
Année de Publication: 2016
Entité/Laboratoire Autres laboratoires
Document type : Article
Discipline(s) : Pharmacie, Pharmacologie & Toxicologie
Titre Activity of red wine polyphenols on endothelial nosynthase (eNOS)
Journal: African Journal of Pharmacy and Pharmacology
Catégorie Journal: Africaine
Impact factor: 0
Volume Journal: 10
DOI: 10.5897/AJPP2015.4491
Resume Polyphenols (PPH) represent a group of chemical substances found in plants, characterized by the presence of more than one phenol unit. The largest and best studied polyphenols are the flavonoids. Polyphenols contained in red wine have beneficial effects on cardiovascular health. The aim of our research was a comparative and interactive study of four red wine polyphenols - resveratrol (R), quercetin (Q), kaempferol (K) and isorhamnetin (IH) which may have a long term properties as the increase of (NO) synthase expression, acting on the prometer activity when used alone or in equimolar mixtures. To determine their activity, we performed a luciferase reporter gene assay on EA.hy926 cells stably transfected with a luciferase reporter gene construct containing eNOS promoter. The Bradford assay was also performed to evaluate the cytotoxicity and the differences in cell number. The median effect equation, as an interaction analysis evaluating synergy or antagonism of the combinations was done according to the principle of mass-action law and the dose reduction index (DRI) was calculated for all mixtures. All single polyphenols activated eNOS promoter. The EC50 values were in micromolar concentrations ranging from 3.44 μM (R2 = 0.96) for kaempferol to 9.89 μM for isorhamnetin (R2 = 0.94). All mixtures activated eNOS promoter, but their interactions varied from synergy (Q+R, Q+IH+K, Q+R+K and Q+R+IH+K), through additive (R+IH+K) to antagonistic interaction (R+IH, R+K, Q+IH, Q+K, IH+K and R+Q+IH). In this study, we show for the first time that red wine polyphenols activated eNOS promoter when used alone and in mixtures with different types of interactions.
Mots clés Polyphenols, eNOS promoter, luciferase reporter gene assay, interaction analysis
Pages 766 - 771
Fichier (PDF)

Publications par entité

  • Centre de Formation et de Recherche en matière de Population (CEFORP) (0)
  • Centre Interfacultaire de Formation et de Recherche en Environnement pour le Développement Durable (CIFRED) (0)
  • Chaire Internationale en Physique Mathématique et Applications (CIPMA-Chaire UNESCO) (0)
  • Ecole Doctorale Pluridisciplinaire <<Espaces, Culture et Développement>> (0)
  • Ecole Nationale d'Administration et de Magistrature (ENAM) (0)
  • Ecole Nationle d'Economie Appliquée et de Management (0)
  • Ecole Normale Supérieure (0)
  • Ecole Polytechnique d'Abomey-Calavi (EPAC) (0)
  • Faculté de droit et de Science Politique (FADESP) (0)
  • Faculté des Lettres et Sciences Humaines (FLASH) (0)
  • Faculté des Lettres, Langues, Arts et Communications (FLLAC) (0)
  • Faculté des Sciences Agronomiques (0)
  • Faculté des Sciences de la Santé (FSS) (0)
  • Faculté des Sciences Economiques et de Gestion (FASEG) (0)
  • Faculté des Sciences et Techniques (FAST) (0)
  • Faculté des Sciences Humaines et Sociales (FASHS) (0)
  • Institut de Formation et de Recherche en Informatique (IFRI) (0)
  • Institut de Mathématiques et de Sciences Physiques (IMSP) (0)
  • Institut National de l'Eau (INE) (0)
  • Institut National des Métiers d'Art d'Archéologie et de la Culture (INMAAC) (0)
  • Institut National Médico Sanitaire ( INMeS) (0)
  • Institut Régional de Santé Publique (IRSP) (0)

Publications par sexe

  • Femme( 0 )
  • Homme ( 0 )

Publications Globales

  • Total publications(0)