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[ Article ] Assessment of genetic diversity among cultivated Pearl millet (Pennisetum glaucum, Poaceae) accessions from Benin, West Africa.

Date de soumission: 12-02-2018
Année de Publication: 2017
Entité/Laboratoire
Document type : Article
Discipline(s) :
Titre Assessment of genetic diversity among cultivated Pearl millet (Pennisetum glaucum, Poaceae) accessions from Benin, West Africa.
Auteurs ADEOTI ADEOLA ZOURI-KIFOULI [1], DJEDATIN LAMBERT GUSTAVE [2], EWEDJE EBEN-EZER BABA KAYODE [3], BEULE Beule [4], SANTONI Sylvain [5],
Journal: African Journal of Biotechnology
Catégorie Journal: Africaine
Impact factor: 0.573
Volume Journal: 16
DOI: DOI: 10.5897/AJB
Resume Simple sequence repeat (SSR) molecular markers were used for genetic diversity analysis and population structure of the cultivated Pearl millet in Benin, West Africa. In order to assess the level of genetic diversity, 14 polymorphic SSR markers were used to screen 114 accessions from different agroecological zones in Benin. SSR markers were found to reveal a total of 57 alleles with an average of 4.071 allele per locus. Genetic diversity index varied from 0.099 to 0.633 with an average of 0.405. The average observed heterozygosity was found to reach 0.425. The analysis of molecular variance showed no real differentiation between regions. Only 5% of genetic variation was observed between samples collected from north-eastern and north-western region. A high level of variation (95%) was observed among accessions. Moreover, both principal component analysis (PCA) and the dendrogram obtained from the genetic distance among accessions revealed the absence of any specific structuration of accessions from each region under study. Our results confirmed diversity among cultivated Pearl millet in Benin and such diversity is not clustering according to geographical patterns.
Mots clés Cereal, simple sequence repeat (SSR) markers, genetic variability
Pages 782 - 790
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