| Resume |
The effects of activated charcoal were tested on medium term conservation of yams cultivated through
tissue culture techniques. Galzy glutamine basic medium and that supplemented with 3 g/l activated
charcoal were used. Growth parameters such as number of leaves, height of stem, number of nodes
and length of the main root are evaluated on plantlets after 20 months. The test T of Student Newman
and Keuls (SNK) with two independent variables was used for the comparison of means with Minitab 16
software. Plantlets obtained in the control medium faded and presented a high number. The length of
internodes obtained from the medium treated with activated charcoal had a length relatively greater
than the control medium with increase of 33%. Probabilities (P = 0.001) associated with the T-test of
equality of means of leaves, the height of stem and the length of internodes are lower than 0.05 but
there was no significant difference between the number of nodes, length of internodes, number of
roots, and length of the main root of plantlets obtained in both media at 5%. In addition, tests of
correlation (Pearson) in the control medium revealed the existence of a highly significant link (P <0.001)
between the number of nodes and number of leaves. In treated medium, there was a highly significant
link (P <0.004) between the length of the stem and number of leaves; the height of the stem and length
of the main root (P <0.001). Activated charcoal facilitates conservation and constitutes an alternative of
conservation of yam. These results may be used to realize in vitro collection of different genotypes of
yam cultivated in Benin. |