||Effects of system benzylaminopurine-adenine sulphate in combination with naphthalene acetic on in vitro regeneration and proliferation of pineapple (Ananas comosus (L.) Mill var. comosus).
AGBIDINOUKOUN ARNAUD ,
||American Journal of Biotechnology and Bioscience
||In vitro micropropagation is now used in the production of
healthy and homogenous pineapple planting materials. In order
to increase the in vitro proliferation of pineapple, the synergetic
effect of adenine sulphate (AdS) and benzylaminopurine (BAP) in
presence or not of naphthalene acetic acid (NAA) was evaluated.
Median axillary buds of crowns bursted 6 to 8 weeks old were
cultured on Murashige and Skoog medium supplemented with
BAP (0, 1 and 2 mg/l), NAA (0 and 0.5 mg/l) with or without AdS
(40 mg/l). Five buds per treatment with four replicates were used.
The addition of BAP to the medium stimulated regeneration (65
to 82%) and multiplication (3.04 to 6.13 plantlets/explant) which
were further enhanced by the addition of NAA (98%; 6.42 plantlets/
explant). AdS highly stimulated proliferation in presence of
2 mg/l BAP and NAA (14.96 plantlets/explant), plantlet mass
(1.143g) but limited plantlet growth in height which was more induced
in the absence of regulators (4.88 cm) and with AdS alone
(5.63 cm). Thus, BAP is necessary for the proliferation of pineapple
and the addition of 0.5 mg/l NAA to 2 mg/l BAP and 40
mg/l AdS optimizes the multiplication and growth parameters of
pineapple plantlets. These results allow the mass propagation of
pineapple’s homogeneous healthy planting materials.
||Pineapple, micropropagation, adenine sulphate,
benzylaminopurine, naphthalene acetic acid, shoots.
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