||Background: Acacia ataxacantha (Fabaceae), used in traditional medicine grows in the South-West of Bénin. Ethyl
acetate extract of the barks of this species was previously reported to display various bioactivities, including
antibacterial, antifungal and antioxidant activities. In the present study, we investigate the antimicrobial and
antioxidant activities of compound isolated from ethyl acetate extract of Acacia ataxacantha.
Methods: Purification, isolation and structural identification of isolated compound were done using various
chromatographic and spectroscopic methods. Antimicrobial activity was investigated using a two-fold serial
microdilution method. The inhibitory potency of isolated compound was evaluated by kinetic experiments. The
antioxidant activity was also determined using 2, 2-diphenyl-1-picrylhydrazyl.
Results: The isolated compound was identified as 7-hydroxy-2-methyl-6-[β-galactopyranosyl-propyl]-4H-chromen-4-
one. As far as we know, this compound, named “acthaside”, reported for the first time, was active against all tested
microorganisms with minimal inhibitory concentration ranging from 25 to 50 μg/ml. At 50 μl/ml, no growth was
observed in almost all tested microbial after 24 h of exposure. The isolated compound had significant antioxidant
activity with an IC50 value of 3.61 ± 0.12 μg/ml compared to quercetin (IC50 1.04 ± 0.01 μg/ml).
Conclusion: The present work demonstrates that the new chromen derivative isolated from A. ataxacantha may
help treat bacterial and yeast infections. However, further studies are required to clarify the mechanism of action of