Publications Scientifiques

[ Article ] Molecular basis of permethrin and DDT resistance in an Anopheles funestus population from Benin

Date de soumission: 01-02-2021
Année de Publication: 2018
Entité/Laboratoire
Document type : Article
Discipline(s) : Parasitologie
Titre Molecular basis of permethrin and DDT resistance in an Anopheles funestus population from Benin
Auteurs Tchigossou Genevieve M [1], DJOUAKA Rousseau [2], Akoton Romaric [3], Riveron Jacob [4], Irving Helen [5], Atoyebi Seun M [6], MOUTAIROU KABIROU [7], YESSOUFOU AKADIRI [8], Wondji S. Charles [9],
Journal: Parasites & Vectors
Catégorie Journal: Internationale
Impact factor: 2.824
Volume Journal: 11
DOI: doi: 10.1186/s13071-018-3115-y
Resume Background: Insecticide resistance in Anopheles mosquitoes is threatening the success of malaria control programmes. In order to implement suitable insecticide resistance management strategies, it is necessary to understand the underlying mechanisms involved. To achieve this, the molecular basis of permethrin and DDT resistance in the principal malaria vector, Anopheles funestus from inland Benin (Kpome), was investigated. Results: Here, using a microarray-based genome-wide transcription and qRT-PCR analysis, we showed that metabolic resistance mechanisms through over-expression of cytochrome P450 and glutathione S-transferase genes (GSTs) are a major contributor to DDT and permethrin resistance in Anopheles funestus from Kpome. The GSTe2 gene was the most upregulated detoxification gene in both DDT- [fold-change (FC: 16.0)] and permethrin-resistant (FC: 18.1) mosquitoes suggesting that upregulation of this gene could contribute to DDT resistance and cross-resistance to permethrin. CYP6P9a and CYP6P9b genes that have been previously associated with pyrethroid resistance were also significantly overexpressed with FC 5.4 and 4.8, respectively, in a permethrin resistant population. Noticeably, the GSTs, GSTd1-5 and GSTd3, were more upregulated in DDT-resistant than in permethrin-resistant Anopheles funestus suggesting these genes are more implicated in DDT resistance. The absence of the L1014F or L1014S kdr mutations in the voltage-gated sodium channel gene coupled with the lack of directional selection at the gene further supported that knockdown resistance plays little role in this resistance. Conclusions: The major role played by metabolic resistance to pyrethroids in this An. funestus population in Benin suggests that using novel control tools combining the P450 synergist piperonyl butoxide (PBO), such as PBO-based bednets, could help manage the growing pyrethroid resistance in this malaria vector in Benin.
Mots clés Anopheles funestus; DDT; Insecticide resistance; Kpome; Permethrin; Resistance mechanisms
Pages 602 - 610
Fichier

Publications par entité

  • Centre de Formation et de Recherche en matière de Population (CEFORP) (21)
  • Centre Interfacultaire de Formation et de Recherche en Environnement pour le Développement Durable (CIFRED) (43)
  • Chaire Internationale en Physique Mathématique et Applications (CIPMA-Chaire UNESCO) (45)
  • Ecole Doctorale Pluridisciplinaire <<Espaces, Culture et Développement>> (0)
  • Ecole Nationale d'Administration et de Magistrature (ENAM) (73)
  • Ecole Nationle d'Economie Appliquée et de Management (88)
  • Ecole Normale Supérieure (35)
  • Ecole Polytechnique d'Abomey-Calavi (EPAC) (1126)
  • Faculté de droit et de Science Politique (FADESP) (114)
  • Faculté des Lettres et Sciences Humaines (FLASH) (1381)
  • Faculté des Lettres, Langues, Arts et Communications (FLLAC) (0)
  • Faculté des Sciences Agronomiques (1943)
  • Faculté des Sciences de la Santé (FSS) (2339)
  • Faculté des Sciences Economiques et de Gestion (FASEG) (190)
  • Faculté des Sciences et Techniques (FAST) (1082)
  • Faculté des Sciences Humaines et Sociales (FASHS) (70)
  • Institut de Formation et de Recherche en Informatique (IFRI) (76)
  • Institut de Géographie de l'Aménagement du territoire et de l'Environnement (IGATE) (0)
  • Institut de Mathématiques et de Sciences Physiques (IMSP) (124)
  • Institut National de l'Eau (INE) (28)
  • INSTITUT NATIONAL DE LA JEUNESSE DE L’EDUCATION PHYSIQUE ET SPORT(INJEPS) (241)
  • Institut National des Métiers d'Art d'Archéologie et de la Culture (INMAAC) (11)
  • Institut National Médico Sanitaire ( INMeS) (0)
  • Institut Régional de Santé Publique (IRSP) (84)

Publications par sexe

  • Femme( 0 )
  • Homme ( 0 )

Publications Globales

  • Total publications(0)