||Antibiotics Resistance and Biofilm Formation Capacity of Staphylococcus spp. Strains Isolated from Surfaces and Medicotechnical Materials.
Socohou Akim ,
SINA HAZIZ ,
DEGBEY CYRIAQUE C. ,
Nanoukon chimène ,
CHABI-SIKA KAMIROU ,
Ahouandjinou Hélène ,
Lehmane Halfane ,
BABA-MOUSSA FARID ,
BABA-MOUSSA LAMINE ,
||International Journal of Microbiology
||Staphylococcus spp. is most often implicated in nosocomial infections. ,e objective of this study is to evaluate the susceptibility to
antibiotics and the biofilm formation capacity of staphylococci species isolated from surfaces and medicotechnical materials at the
university hospital center of Abomey-Calavi/Sˆo-Ava in Benin. Samples were collected according to ISO/DIS14698-1 standard
from the surfaces and medicotechnical materials by the dry swab method. ,e isolation of Staphylococcus strains was performed
on Chapman agar, and their identification was performed using microscopic and biochemical methods. ,e susceptibility of
Staphylococcus isolates to antibiotics was evaluated by the disc diffusion method according to EUCAST and CLSI recommendations.
,e biofilm formation was qualitatively assessed using microplates. Of the 128 surfaces and medicotechnical material
samples analyzed, 77% were contaminated with Staphylococcus spp. ,irteen species of Staphylococcus were isolated in different
proportions but the pediatric department was the most contaminated (33%) by S. aureus. Resistance to antibiotics considerably
varies according to the species of Staphylococcus. However, antibiotics such as chloramphenicol and vancomycin are the most
effective on S. aureus, whereas coagulase-negative staphylococci developed less resistance to gentamycin and ciprofloxacin. ,e
biofilm test reveals that 37% of our isolated strains were biofilm formers. Although regular monitoring of hospital hygiene is
crucial, the optimal use of antibiotics is a cornerstone of reducing antimicrobial resistance.
||Hospital hygiene, Staphylococcus spp., Antibiotic resistance, Biofilm, Medico-technical materials, Benin.
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