||Aims: This study was designed to assess antiplasmodial and antioxidant activities in relation to
phytochemical contents and toxicological profile of crude extracts of Cola millenii leaves.
Place and Duration of Study: The study was carried out from April 2015 to November 2018 at
University of Abomey-Calavi, Laboratory of Biochemistry and Bioactives Natural Products and
Laboratory of Infectious Vector-borne Diseases.
Methodology: Phytochemical screening of aqueous and ethanolic crude extracts was performed
using standard methods. Estimation of total phenolics contains (TPC) and total flavonoids contains (TFC) was done using colorimetric methods. Antioxidant activity was assessed in vitro by 2,2-
diphenyl-1-picrylhydrazyl (DPPH) Radical-Scavenging, Reducing power, superoxide radical
scavenging and Hydrogen peroxide scavenging assay. Antiplasmodial activity was investigated in
vitro using both field isolate and laboratory 3D7 strains of P. falciparum using Plasmodium lactate
dehydrogenase (pLDH) assay. Hemoglobin release was measured spectrophotometrically to assess
hemolytic power. Acute oral toxicity of extracts was evaluated on rats according to OECD 423
Results: Flavonoids, anthraquinones, coumarins, and triterpenes were recorded in both extracts
while tannins were recorded only in ethanolic extract. High TPC and TFC were recorded with
ethanolic extract with significate difference (P <0.01) when compared to aqueous extract. Ethanolic
extract exhibited the highest parasite growth inhibition against both field isolate (41.62±1.78%) and
3D7 (45.89±1.66%) strains at 100 μg/mL. Both extracts exhibited strong antioxidant activity
according to different methods used. Toxicological profile revealed no hemolytic effect on human
red blood cells as well as acute toxicity signs in rats.
Conclusion: This study demonstrated strong antioxidant and moderate antiplasmodial activities of
C. millenii extracts without toxicity effect on rats and human erythrocytes. It would play an important
role in malaria and oxidative damage control.